In this study, we systematically interrogated the changes in chromatin structure of human subtelomeres upon cellular senescence.
To understand the signal transduction changes that accompany the cell-death-inducing perturbation of glucose addiction, Graham et al (2012) used a mass spectrometry-based approach to interrogate the changes in the phosphotyrosine proteome that follow glucose deprivation.
In our FPI-FS, the length of FP cavity changed with force and we used a common-path Fourier domain optical coherence tomography (CP-FD-OCT) to interrogate the change of cavity length by phase-sensitive detection.
Mouse models overcome some key hurdles for biomarker discovery, including technology development [16]; more precise matching of cases and controls to reduce genetic, age, and environmental variables; interrogating the dynamic changes in the plasma proteome through disease progression; and integrating results with the extensive biological knowledge bases [17].
To interrogate this possibility, we examined the sensitivity of pStat3 gradients to the changes in expression of gp130-Jak-Stat pathway components in silico.
They will be interrogated regarding changes in their usual symptomatology (expectoration, cough, dyspnea) as well as in the treatment for their respiratory disease.
Genome-wide technologies are commonly used to interrogate transcriptional changes in discovery-based approaches.
This is the first time that, to our knowledge, this technique has been used to interrogate transcriptional changes in cartilage ageing and, importantly, validation studies using RT-PCR demonstrated high correlation between methodologies and demonstrated reproducibility using a different donor set.
The promoters with transgenerational differential methylation were analyzed in the online algorithm Repeat Masker in order to interrogate changes in the representation of particular repetitive elements.
High-dimensional flow cytometry, and more recently mass cytometry, have been exploited to interrogate changes in the cellular compositions before and after vaccination [ 10].
