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We further interrogated the functions of ALDH2, CCNE1 and SMAD3 by searching genes that are functionally similar, or have shared properties using GeneMANIA [ 23].
We next interrogated the functions of genes that were share, or specific in one of the two cancers.
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To identify cancer driver genes in this region, we interrogated the function of each of these genes by loss- and gain-of-function genetic screens.
To learn more about these nine miRNAs, we interrogated the function of their homolog in C. elegans.
In addition, we further interrogated the function of a particular epigenetic regulator in mouse and human pre-implantation development.
We interrogated the function of disrupted meiotic cDNA1 (DMC1), a homolog of RAD51, the primary recombinase used in mitotic cells to search and recombine with the homologous DNA template.
Based on the elevated cellular density in GBM, which increases the opportunity for direct cell communication, and the dependence of CSC maintenance on cell-cell interactions, we interrogated the function of connexins in GBM.
Thus, the thymocyte differentiation assay allows us to interrogate the functions of mir-181a-1 mir-181a-1 mir-181a-1siologicandy relevant mRNA and miRNA milinu.
The Dobbelstein lab therefore performed a comprehensive siRNA screen to interrogate the functions of known ubiquitin ligases and deubiquitinating enzymes in the DNA damage response to cisplatin treatment.
One of the next exciting challenges to our understanding of how complex behaviors are specified at a molecular-genetic level will be to develop tools to interrogate the functions of specific transcript isoforms in a cell-specific manner.
To generate tools to specifically interrogate the functions of GPN2 and GPN3 we created mutant alleles of each gene using a mutagenic PCR approach described previously (Ben-Aroya et al. 2008).
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