Sentence examples for interrogated sequence from inspiring English sources

The C t) value is inversely proportional to the amount of each methylated locus in the PCR reaction well, such that a low C t) value suggests that the interrogated sequence is highly methylated.

Forward pyrosequencing primers, the interrogated sequence and dispensation order are listed in Additional file 2: Table S3.

The abundance of each fragment (signal/noise level in the spectrum) detected by the MassARRAY® system is indicative of the amount of DNA methylation in the interrogated sequence.

Given that the DNA methylation pattern in the entire interrogated sequence, and not just the primer binding sites, influences PCR bias [ 20], eliminating bias in a heterogeneous population of alleles becomes nearly impossible.

To calculate the distance of the target from the 3' end of the interrogated sequence, the target sequence for each probe set (supplied by Affymetrix; was BLASTed [ 16, 17] against the nr (non-redundant) database at NCBI.

All the probes targeting pop-diff CpGs with SNPs in the interrogated sequence belonged to the class flagged to be discarded from methylation analyses by Naeem et al. [ 19].

The first type of probemask excluded all probes except those that interrogated sequences that we confirmed were identical in XL and XB, as in [9], [25].

We interrogated DNA sequence motifs to identify the mechanisms underlying synergistic binding of Hoxa2 and Meis in the IIBA.

The tandem hybridization method described here offers several advantages over the traditional oligonucleotide array configuration in which each interrogated target sequence is represented by a single surface-tethered probe.

After normalization step, probes related to X and Y chromosomes were removed as well as those containing a SNPs with a frequency > 1% (Caucasian population; 1000 Genome project) in the probe sequence or interrogated CpG site.

The U and M probe sequences, complementary to the interrogated CpG and to the downstream genomic sequence, differ both at their 3′ ends (complementary to the targeted CpG site) and at all positions complementary to other neighboring CpG sites covered by the probe sequence.