The loci interrogated included the promoter region of the DNA repair genes: MGMT, MLH1, ATM, BRCA1 and ERCC1 [ 6, 9, 10, 17– 20]; genes mediating control of cellular proliferation: RASSF1A, APC and RUNX3 [ 8, 9, 21– 23]; the pro-apoptotic tumor suppressor gene: DAPK1 [ 6, 9, 10, 24] ; and genes involved in invasion and metastases: CDH1, CDH13 and ABO [ 9, 24– 27].
Fungal genome datasets interrogated included those at the Broad Institute http://www.broad.mit.edu/ (A.
He said he managed to build a rapport with the Japanese he interrogated, including one he said wrote him a letter after the war in which he referred to himself as Dr. Keene's first P.O.W. LIKE several of his classmates, Dr. Keene used his language skills after the war to become a pioneer of academic studies of Japan in the United States.
Overall, using AFLP, 2.08% of the 4,034,664-bp V. cholerae genome [ 29] was interrogated, including 81,408 nucleotides for EcoRI- MseI internal length variation (318 fragments × 256 bp) and nearly 2,862 for point mutations (318 fragments × 9 bp).
Press reports claim that a handful of local Emiraati executives have also been interrogated, including Sami al-Hashemi, ex-CEO of real-estate developer Mizin, and Abdul Salam al-Marri, head of the Lagoons development on Dubai Creek.
Sequence databases from three sponge species were interrogated, including two demosponges (Sponge1, Ephydatia muelleri and Sponge2, Amphimedon queenslandica) and a homoscleromorph sponge (Sponge3, Oscarella carmela).
The downstream pathways interrogated included Ras, Akt, Notch, Wnt and SHH.
Examples of sources of data that may be interrogated include: ambulance dispatches; general practitioner patient notes; and emergency department (ED) provisional diagnoses.
The 179 drug resistance associated genomic positions were interrogated with KvarQ, including the 81 bp Rifampicin Resistance Determining Region (RRDR) of rpoB, the 63 bp Quinolone Resistance Determining Region of gyrA (QRDR) and 3 bp in the codon 315 of katG (Additional file 1).
Because of the high read depth generated, next generation sequencing methods provide an opportunity to interrogate variant data, including the presence of low-level resistance mutations.
