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Recently Harbig et al. used the BLAST program to interrogate the sequence of all the U133Plus2.0 probes against the human genome [ 17].
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To better understand the distinct sources and mechanisms that might facilitate a phenotypic diversity of this magnitude, we comprehensively interrogated the sequence of the SLC6A3 gene applying computational methods.
We have interrogated the sequence of events that involve the apoptotic signalling pathway induced by E2 in comparison with paclitaxel.
However, recent advances in DNA sequencing technology has made it possible to interrogate the sequence space of an organism or community to recover large numbers of putative coding regions in a high throughput manner without the need for laboratory culturing and propagation [ 5].
We therefore interrogated the sequences of the eIF4A-dependent and eIF4A-independent 5′UTRs for the potential to form these structures.
The present study set out to determine the extent and nature of sequence diversity in genes encoding the Tp1 and Tp2 antigens in a series of isolates of T. parva obtained from cattle and buffalo in regions of Africa where ECF is endemic, and to interrogate the sequence data for evidence of the mode and strength of selection.
The SignalP 4.1 and SecretomeP 2.0 servers were used to interrogate the protein sequences of highly over-expressed genes (Table 3) to search for predictions of protein secretion by classical signal peptide – mediated mechanisms or the unconventional pathway which mediates leaderless secretory proteins.
Software tools to find these variants deploy different mathematical approaches to interrogate the genome sequences of tumour/germline paired samples.
Each sequencing lane interrogated the DNA sequences of a pool of three individual sample libraries each carrying a unique index.
To determine whether other maternally imprinted genes are methylated in abnormal sperm, we used MethyLight to interrogate the differentially methylated sequence of DIRAS3.
The GeneChip® Human Mitochondrial Resequencing Array 2.0 (Affymetrix) was used to interrogate the entire mtDNA sequence of the 19 individuals found to carry the m.1555A>G mutation.
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