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The custom cotton microarray contains 283,000 features that interrogate the relative expression intensity of 42,430 unigenes using an average of seven distinct probes per unigene.
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CE-TOF-MS was used for interrogating the relative levels of metabolites between peaks and troughs in PACTIN-PxRe oscillations.
For these studies we initially interrogated the relative elafin mRNA expression in 48 breast cancer cell lines and two HMECs using a microarray dataset [ 23].
We interrogated the relative expression of miR-31, miR-184, miR-17 and miR-19a in a panel of human breast cancer cell lines.
We interrogated the relative mRNA expression of ADRB2 (β2-AR) in human breast cancer tissue samples by searching a publicly available database Oncomine (www.oncomine.org).org
As the initial assessment of PA-seq data, we interrogated the relative location of experimentally identified PA sites (all uniquely mapped pairs) in respect to PolyA_DB annotations [ 14].
Altogether, these experiments showed that OTU DUBs can be used to interrogate the type and relative abundance of Ub chains on substrates.
Intracellular staining (ICS) [ 9], which requires blocking protein secretion and fixing the cells, can be coupled with cytometry to interrogate for the relative levels of functional proteins such as cytokines or phospho-kinases.
To interrogate the factors that determine the relative utilization of the Bloch and MK R pathway, we examined two cell culture systems in which rates of cholesterol biosynthesis were altered.
For future analyses, we recommend that an HPLC or UPLC separation approach be employed rather than 2DGE to interrogate the Phaseolus proteome because of the speed, throughput, and relative cost of the LC-based platform.
However, current methods are limited to analysis of the relative expression level of only a few hundred to perhaps 2000 proteins, well below the ability of DNA microarrays to potentially interrogate the mRNA expression of more than 25 000 genes.
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