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A primary aim here is to complicate (and thereby interrogate) the importance we attach to speakers rather than content, not -- as would be an entirely opposite ambition -- to re-conceive of content as irrelevant.
Therefore, VavNULL mice provided us with a unique tool to interrogate the importance of ITAM-dependent signals in neutrophil motility, as VavNULL neutrophils show a nearly complete block in ITAM signaling, but can still undergo integrin activation via inside-out signaling and undergo chemotaxis in response to chemotactic gradients [8].
To further interrogate the importance of the full-length ProcA leader peptide, we made a series of ProcA2.8 mutants lacking 30, 40, and 50 N-terminal amino acids and coexpressed these mutants with ProcM in E. coli.
In addition, the generality of the solid-phase synthetic methodology should facilitate the construction of other sulfopeptide and sulfoprotein libraries to interrogate the importance of Tyr sulfate modifications in a number of diverse systems, including chemokine-chemokine receptor interactions (e.g. CCR5/RANTES), decoy receptors, and complement proteins, in the future.
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We addressed this question by interrogating the importance of Myc protein levels for transferrin uptake.
Also, we interrogated the importance of the spacer length between the amino group and the phenyl ring using another set of tyramine analogues.
Short hairpin RNA-based and dominant-negative expression-based SHP2 inhibition techniques were used to interrogate the functional importance of SHP2 in BTBC cell biology.
Among other things, Nisman wanted to interrogate the President.
Let's interrogate the role of race in these situations.
Don't interrogate the person.
Don't interrogate the other person.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com