qPCR was used to interrogate five candidate genes (chosen at random) associated with CpG islands, four of which exhibited altered expression in ObC relative to LnC as predicted by the array (Dbp, Gm129, Gna14, Zbtb16; Fig. S3 ).
Therefore, it is essential to interrogate these candidate genes subsequently with other pathological features and validate them systematically by PCR or other independent experimental approaches before utilising them as useful clinical biomarkers.
Public proteomic and gene expression data were used to further interrogate the candidate biomarkers from our secretome analyses to determine whether the secretome from human ovarian cancer cell lines represents a rich source for candidate secreted or shed proteins with biological and translational potential as candidate circulating biomarkers.
The supervisory board screening candidates went so far as to send the basij, the Islamic militia, to interrogate the candidates' neighbours to collect evidence.
The modification helped us in interrogating 29 candidate sequences with the same P – labeled total MAR DNA probe on a single blot.
In Case 3, whole exome sequencing data were interrogated for candidate genes causing parkinsonism dystonia, thereby revealing a homozygous change (c.941C>T; Ala314Val) in SLC6A3.
There were two moderators, one of whom interrogated the candidates through a wisp of a headset mic.
"Donald Trump may be good for ratings, but in its determination to court him, TV news has sacrificed its duty to interrogate the Republican candidate and his policies," wrote Lloyd Grove at the Daily Beast.
Public gene expression data was used to further interrogate the four candidate biomarkers from our in vitro secretome analysis that passed immunoblot verification to evaluate the clinical relevance of some of the candidate biomarkers.
Whereas 286 mutations were found by assays interrogating known or candidate oncogenes, 131 mutations were observed in TS genes.
