We interrogated a range of samples including human cell lines, primary tumours and primary tissue samples.
We took a single tissue, articular cartilage, and undertook RNA-Seq in order to interrogate a greater range of genes for differential expression.
Whereas TaqMan-based assays are limited by the efficiency of the oligonucleotide probe and dependent on the neighboring nucleotide context, our single-color digital PCR strategy is a highly flexible platform that can be used to interrogate a wide range of genetic targets.
These sampling strategies are applied to whole three-dimensional organs, well-defined fragments of organs, and ultimately to two-dimensional sections interrogated using a range of stereological probes including points, lines, curves, frames, and optical and physical disectors.
Nucleation inhibition activity using droplet freezing has been interrogated using a range of methods, including microfluidics, suspension in oil, or using electrodynamic balances.
Our study also was the first to analyze the postnatal ontogeny of the innate immune system using ICS in all 4 major APC subsets at the same time in the same sample, and the first to interrogate a wide-range of specific TLR responses using the currently most comprehensive innate profiling platform by coupling flow cytometry with a multiplexed bead array approach.
In this paper we have electrochemically interrogated and characterised a range of commercially available screen-printed electrodes from Zensor, Dropsens and Kanichi with scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).
Future studies utilizing the rapidly increasing power of genome sequencing technologies to interrogate a much wider range of candidate genes, and eventually the whole exome, for rare variants that predispose to CVM will be of great interest.
In addition, several non-antibody protein scaffolds that interrogate a broad range of targets are being pursued.
The ability to interrogate and resolve bacterial populations is applicable to a range of infectious diseases, as well as microbial ecology.
