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"We believe we did well in the DREAM2 transcription factor target challenge, at least in small part, because we adopted a model for interpreting expression data that we knew that the organizers themselves subscribed to.
Given that many of the genes encode products of unknown function, we cannot offer a functional rationale for these observations other than to suggest caution when interpreting expression changes for these genes in responses of cells with the Δ pyrF genotype.
The complexity of the healthy haematopoietic system is immense, and as such, one must understand the biology driving normal haematopoietic expression profiles when designing experiments and interpreting expression data that involve normal cells.
This would make interpreting expression patterns for those genes quite difficult in composite extractions.
Use of a fluorogen with FISH, rather than a chromogen as in IHC, means that interpreting expression can be more straightforward than with IHC [ 68].
These data suggest caution in interpreting expression alone as directly proportional to functional activity in clinical samples, and clearly show that Bcl-2 and Bcl-XL are functionally distinct.
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Like the countries, the Internet companies have their own positions, which give them wide latitude on how to interpret expression in different countries.
The FiDePa algorithm interprets expression differences between tumor and normal tissue and relies on GSEA.
We used "level 3" data which corresponds to pre-processed and interpreted expression signals for each gene.
We also see a cluster with a less easily interpreted expression pattern (Supplementary Material, Supplementary Fig. S5B, cluster 4).
In order to better understand the functional relevance of altered MMP expression and the exact involvement of these endopeptidases in GON, mechanistic studies are needed to interpret expression data obtained in GON patients.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com