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The results obtained were interpreted as sensitive or resistant according to the National Committee on Clinical Laboratory guidelines [18].
In general for the 3 loci, a pattern comprising only WT bands was interpreted as sensitive.
Finally, the diameter of growth inhibition around the discs was measured and interpreted as sensitive (S), and resistant (R) as per the guidelines of the manufacturer.
The diameters of the zones of inhibition were recorded and interpreted as sensitive, intermediate sensitive or resistant, according to the CLSI guidelines 2013 [ 4] except colistin and tigecycline where in CLSI guidelines for Acinetobacter is not available.
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Discrepancies were differentiated into three categories, minor (susceptible 'S' interpreted as less sensitive 'LS'LS'LS' as 'S' or resistant 'R' and 'R' as 'LS'), major ('S' interpreted as 'R') and very major ('R' interpreted as 'S'), which are defined by US Department of Health and Human Services Food and Drug Administration.
Each measurement of the inhibition zone diameter was interpreted as 'sensitive'intermediateate' or 'resistant' according to CLSI standard interpretative charts [ 25].
Clinical breakpoint for penicillin of ≤ 2 mg/L and for trimethoprim-sulfamethoxazole of ≤ 0.5/9.5 mg/L is interpreted as 'sensitive' as per this document.
The two "extreme" patterns of drug susceptibility (fig. 1a and 1f) may be easily interpreted as drug-sensitive and drug-resistant, respectively.
The results were interpreted as resistant (R), intermediate sensitive (I) and sensitive (S) as described by Clinical and Laboratory Standard Institute CLSII formerly NCCLS) guidelines 2007 [ 10].
Therefore, superinfection at 39°C can be interpreted as a temperature-sensitive effect on HN 262+264 binding activity.
The diameter (in millimeters) of the zone of inhibition was measured and interpreted as resistant, intermediate or sensitive using CLSI guidelines for the respective antibiotic [ 19].
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