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The MIC (μg/mL) interpretative standard for vancomycin and teicoplanin were those suggested by EUCAST (respectively: ≤ 4 susceptible, ≥ 8 resistant).
Antimicrobial susceptibility testing (AST) was performed using the E-test method on Mueller-Hinton agar supplemented with 5%% sheep blood (BioMérieux, Marcy l'Etoile, France) incubated at 36 °C in 5 % CO2 for 24 h according to the Clinical and Laboratory Standards Institute (CLSI) interpretative standard for Streptococcus species [ 8].
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We therefore conclude, that susceptebilty testing should be routinely performed, when A. schaalii is detected in relevant clinical specimens and interpretative standards for interpretation of MICs should be developed.
Antimicrobial sensitivity test results were interpreted according to the interpretative standards of the National Committee for Clinical Laboratory Standards (NCCLS) [ 12, 13, 17- 19, 21, 22] and the Clinical Laboratory Standards Institute (CLISI) [ 20].
Antibiotic sensitivity was considered according to the zone diameter interpretative standards of CLSI [ 26].
Susceptibility testing was performed using Vitek2 microbroth dilution according to EUCAST interpretative standards [ 23].
Beguelin et al. described the results of antimicrobial resistance testing in their study, although there are no recognized minimal inhibitory concentration (MIC) interpretative standards for A. schaalii [ 10].
According to the Clinical and Laboratory Standards Institute MIC interpretative standards for staphylococci (10 ), antibacterial agent susceptibility testing revealed resistance to benzylpenicillin and oxacillin/cefoxitin for all isolates.
According to the interpretative standards for Burkholderia cepacia, the epidemic strain was resistant to piperacillin (>256 μg/mL), piperacillin-tazobactam (>256 μg/mL), ticarcillin-clavulanic acid (>256 μg/mL), ceftazidime (>256 μg/mL), cefepime (>256 μg/mL), imipenem (>32 μg/mL), meropenem (>32 μg/mL), ciprofloxacin (>32 μg/mL) and colistin (>256 μg/mL).
All strains were tested for antimicrobial susceptibility by MIC determination to tetracycline, trimethoprim/sulfamethoxazole, sulfonamides, ampicillin, chloramphenicol, nalidixic acid, and ciprofloxacin by using Etest (AB bioMérieux, Solna, Sweden) according to Clinical and Laboratory Standards Institute procedures and interpretative standards for V. cholerae (1 ).
The diameter of the zones of inhibition of the isolates to various antibiotics was interpreted using the standard interpretative chart updated according to the current Clinical Laboratory Standard Institute (CLSI) standard and Fluka zone interpretative chart, in accordance with WHO requirements.
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