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The comparisons of normalized expression of the target gene, GPNMB, showed different interpretation of target gene expression depend on best single reference gene or combination.
As a result, the use of GAPDH as a single, unverified reference gene would invariably lead to erroneous interpretation of target gene regulation.
Next, the possibility that using scaling factors of NFRPL19/29, NFACTB or NFGAPDH may substantially alter the interpretation of target gene expression regulation in NGF induced neuronal differentiation was investigated.
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Several problems need to be addressed in future investigations: the lack of studies (and genomic information) on key ecological species and taxa, the need to better understand the different transcriptomic responses to high and low doses and, especially, short and long exposures, and the need to improve experimental designs to minimize false transcriptome interpretations of target genes.
Contrary to prediction, results demonstrated that participants in the Positive CBM-I condition evidenced a significant shift towards more positive interpretations of target scenarios, irrespective of OC status.
During cross-species microarray hybridizations, a sequence mismatch between the probe and the target sequence in one species can lead to inaccurate interpretations of target levels between the species.
However, the interpretation power of target analysis can be magnified significantly if we harness the full informational potential (GCtimes GC): combining the well-known characteristics of target biomarkers (major peaks) with the lesser known nuances of non-target biomarkers (minor peaks), which occupy the breadth of the intricate (GC times GC) topography.
Rev. 98, 708 729) called the interactive context (which affects the meaning, or the interpretation, of the target event) in contrast to the independent context (which does not interfere with the meaning-based interpretation of the target event).
The signature variations may difficult the interpretation of the target locations and orientations in the resulting SAR images.
In many pattern recognition applications, feature selection focuses on identifying the most significant features that have relevance to the interpretation of the target classes.
KEGG pathway analysis was performed for biological interpretation of these target unigenes of differentially expressed miRNAs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com