The ordination approach is more naturally able to account for the difference in information content arising from the non-square data matrices, missing data, and differences in interpretation of matches for categorical variables [25].
However, the comparison of gene expression is most biologically meaningful when the embryos are from similar developmental time points, which means that the use of specific stage would be useful to improving the interpretation of matches.
Peak identification was accomplished through manual interpretation of spectra and matching against the NIST'02 library and comparison with commercially available standard samples when available.
Metabolite identification was accomplished through manual interpretation of spectra and matching against the Agilent MS ChemStation Software (Palo Alto, CA, USA) or commercially available standard samples, when available.
Particularly useful was close communication with the Wadsworth Center on interpretation of spoligotype matches for "rare" spoligotypes (seen less often than average for most spoligotypes in our database) and on prioritization of these isolates for investigation as clusters or false-positive cultures.
The use of matched samples allowed for accurate interpretation of the results with these subject numbers.
The drawback is that these qualities are not delineated, so researchers cannot be certain if their interpretation of the label matches that of the participants.
The interpretation of these patterns matched the information contained in the pairwise comparisons of the microarray data, which finally allowed us to uncover the genes that are regulated by both Hsp90 and p23.
Interpretation of these data is made more difficult by the lack of matched tissue from healthy control subjects.
"Luckily, I work with a great director who helps with the interpretation of the line, matching of the lip flap, and consistency of the voice," she said.
