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Such controls are required for proper interpretation of gene function studies.
Protein overexpression may result in non physiological conditions and thereby lead to incorrect interpretation of gene function.
Identifying the timing of up- or down-regulation can impact the interpretation of gene function or cellular dynamics.
However, cross-reactivity, whereby multiple genes may be simultaneously targeted by a single short interfering RNA (siRNA), can potentially jeopardize correct interpretation of gene function.
Thus accurate interpretation of gene function can only be effectively obtained using genomic DNA (gDNA) clones comprising entire coding regions in expression studies with subsequent analysis of individual splice variants using corresponding cDNAs.
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The BioGRID dataset has been kept current with the literature through archived monthly updates, and has found numerous applications, from the analysis of biological network properties, to predictions of gene function, to the interpretation of genetic interactions, to a standard for automated text mining approaches [ 4].
Better understanding of gene function for these species allows for better interpretation of clinical experiments, leading to an increased understanding of gene roles and potential drug targets.
We provide interpretation of the gene function based on GO terms, the UniProt database, and published literature, although there may be additional functional roles of the identified genes that are not outlined here.
It wants to sell subscriptions to a database of gene functions it is compiling.
In-depth analysis and description of the denoted genes for molecular function and pathological involvements was additionally done using BiblioSphere Pathway Edition (Genomatix, Germany), including a comprehensive manual interpretation of the gene functions by intensive literature study.
The aim of the present work is a clarification of the nomenclature of molluscan p53 family sequences, an essential prerequisite for reliable interpretation of gene expression and protein function studies.
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