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Clearly, the interpretation of binding assays should be done with caution.
Whether these are close enough to justify quantitative interpretation of binding experiments is however questionable.
The stability enhancement averages 60 000-fold, and just as important, the sequence dependence of free energies, compared to unmodified probes, is reduced; this greatly simplifies interpretation of binding results.
A major difficulty comes from the interpretation of binding data measured in complex systems such as total membranes or whole cells from different tissues where other proteins may affect the interaction.
Furthermore human serum albumin has been the preferred albumin and interpretation of binding data for human serum albumin in terms of BSA structure should proceed with caution as both albumins only share 76% sequence homology.
Since these circumstances would have complicated the interpretation of binding studies mediated exclusively via the mesothelin moiety of Meso-TR3, we first saturated the death receptor binding sites of Meso-TR3 with soluble death receptor 5 (DR5-Fc).
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The proposed binding specificity enables a detailed interpretation of RNA binding results obtained for RSV M2-1 by Tanner et al. (2014).
These results show that caution is needed where a mechanistic interpretation of polymer binding is derived from interpreting LTSEM images.
The combined interpretation of the binding and activity data suggests that XOS binds to the active site of TrCel7A and hence exerts competitive inhibition.
An interpretation of RF binding at pattern 3 DMRs with respect to CpG-density is that RF binding occurs in regions of elevated CpG-density, with coordinated methylation changes in adjacent lower CpG-density regions.
The auger interpretation of the binding energy (367.39 eV; Fig. 2c) and the KE of AgMNN (358.8 eV) indicate the existence of metallic silver NPs in our sample, suggesting that the sample has active plasmon resonance and substantial hot electron generation.
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