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In order to interpret the binding of the PKA on RyR2, we performed elastic net analysis of energetically responsive residues of RyR2.
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Since 18∶1 oleic acid acts as the HAMLET co-factor, the amount of 18∶1 fatty acid 18∶1 acyl chains in the liposomes is of interest when interpreting the binding results.
Imaging studies of the aggregation products showed large amounts of amorphous material, which was interpreted as the binding of αB-crystallin to monomeric or oligomeric intermediates, inhibiting the nucleation of fibrillar αSyn aggregates.
As this information was used to interpret the AUC binding experiments of TOG1-TOG2 witubulinlin using different assumption about cooperativity, the authors should provide more details about the exact way these calculations were performed.
These flow-mediated transport mechanisms are also used to interpret the role of binding site densities and injury size on initiating coagulation and fibrin deposition.
Combining these data with additional functional information allows us to interpret the consequences of RNA binding in diverse cellular processes, including alternative splicing, 3′ end processing, and translation (Darnell et al., 2011; Hafner et al., 2010; König et al., 2010, 2012; Licatalosi et al., 2008; Ule et al., 2003; Wang et al., 2012).
We speculate that certain GalNAc-type O-linked glycans may serve as determinants for both CTB recognition and membrane microdomain targeting, potentially explaining why CTB binding fractionates with detergent-insoluble material, an observation that is typically interpreted to result from the binding of CTB to GM1.
In addition to substrate binding sites, it is also important to interpret the possible incidence of mutations at sites of interaction between proteins.
To further interpret the enrichment of axis proteins at 3′ ends of genes, we confined the analysis of binding peaks to 500 bp around Red1 summits.
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