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One exception, as measured by the different test result photographs, was the ability to correctly interpret faint positive test lines as positive.
This paper reviews specific issues surrounding RDT use in field settings and presents results of research investigating how to interpret "faint test bands" on ParaCheck Pf® in areas of low transmission in order to reduce malaria misdiagnosis.
In the current clinical environment where training and experience results in presumptive malaria treatment and belief that "a negative blood slide does not rule out malaria" it is essential for training algorithms to clarify how to interpret "faint bands" occurring in ParaCheck Pf® and potentially other RDTs should be interpreted [ 3].
Discerning how to interpret "faint bands" and the resulting impacts on malaria misdiagnosis in areas of medium to high transmission and may give different results and therefore we advocate for further research that will add to the growing body of knowledge regarding RDTs and their inherent strengths and limitations.
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These results may be due, as the authors suggested, to the lower sensitivity obtained on site by the nurses interpreting faint test lines as negative results.
Given these known difficulties, it seems reasonable to interpret the faint and variable plastid GFP fluorescence observed herein as evidence of dual targeting.
Furthermore, he put aside the apparent faint novae in M31, preferring to interpret S Andromeda as an ordinary nova, for otherwise that object would have been unbelievably luminous.
Besides major bands, faint to moderately strong bands were present in nearly all Southern-RFLP patterns; we interpret these weaker bands as low-copy molecules of mt DNA [ 25, 26].
The third pattern (i.e. faint staining throughout the cell body) persists following pre-incubation of the Rosetteless; we interpret this third pattern then to reflect either autofluorescence or non-specific staining.
Usually they are so faint and masked by static interference that it's hard to make out what they are saying, and the EVP investigator has to "interpret" them for you.
If nurses had interpreted the appearance of a weak, faint test line as positive, this could have resulted in reduced test specificity.
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