Exact(4)
A total of 12 datasets was acquired with each commercially available CZT camera (DNM 530c, GE Healthcare and DSPECT, Biosensors International) using both energy windows (99mTc or 123I) with ejection fraction set to 33, 45, and 60%%.
Samples positive by SimulFluor were tested by DFA Kit (Chemicon International) using individual monoclonal antibodies against RSV, influenza A (INF A) and B (INF B), parainfluenza 1 (PIV 1), 2 (PIV 2), and 3 (PIV 3), and adenovirus.
In order to confirm that the monolayer of fibroblasts that was used in our experiments was also producing FGF-7, we first immunostained the fibroblasts in situ with a monoclonal antibody directed against FGF-7 (Chemicon International), using a procedure that we have previously described in detail [ 13].
Amplification of the spacer DNA was carried out in a Corbett thermal cycler (Biotech International) using 30 cycles of DNA denaturation for 1 min at 96°C; annealing for 1 min at 55°C and elongation for 30 sec at 72°C.
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