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internally transcribed spacer.
This heterogeneity was corroborated by extensive fine-scale variation among A. phosphatis rRNA internally transcribed spacer regions (He et al., 2006) and the polyphosphate kinase 1 gene (He et al., 2007; Wilmes et al., 2008a).
However, internally transcribed spacer region sequencing of strain 313 indicates that it, in fact, belongs to group 1 (D. Warnock, pers. comm).. Alternately, this finding may suggest past genetic exchanges between group I and non group I strains.
BDG: 1-3 -β-D-glucann; 1-3 -β-D-glucan Chain Reaction; DNA: Deoxyribonucleic Acid; ALL: Acute Lymphoblastic Leukemia; AML: Acute Myeloblastic leukemia; KOH: Potassium Hydroxide; KCl: Potassium Chloride; ITS: Internally Transcribed Spacer; CBS: Centraalbureau voor Schimmeculture The authors declare that they have no competing interests.
The internally transcribed spacer (ITS) is the DNA situated between the small-subunit rRNA and the large-subunit rRNA genes in the chromosome or the correspondingly transcribed region in the polycistronic rRNA precursor transcript.
PCR primers were designed to examine three regions of rDNA, i.e., the promoter, the internally transcribed spacer (ITS) and the intergenic spacer (IGS).
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In these subunits, the internal transcribed spacer (ITS) is not internally repetitive and its evolving rate is between those of genes and non-transcribed region [ 11].
3.5 μl of in vitro transcribed, internally digoxigenin-labeled probe were added for overnight hybridization.
In vitro transcribed, internally radiolabeled RNA probes to the entire 3′ untranslated region of Kunjin virus (GenBank: AY274504.1 nts 10,396-11,022) were generated using a template containing the Kunjin 3′ UTR inserted into the pGEM-4 vector (X65303.1; GenBank) in the EcoRI and HindIII restriction sites using these primers: 5′-GAATTCTAAATACTTTGTTAATTGTAAAT; 5'-AAGCTTAGATCCTGTGTTCTCGCACCACCA.
rDNA internal transcribed spacers.
internal transcribed sequences.
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