Sentence examples for internally labeled from inspiring English sources

The EGS in 100-, 50- or 10-fold molar excess was incubated with 10 nM of 5' end-labeled or internally labeled target mRNAs in PA buffer (50 mM Tris-Cl, pH 7.5, 10 mM MgCl2, 100 mM NH4Cl) for 5 min at room temperature.

To evaluate miRFP670nano as an internal tag, we constructed internally labeled G protein α-subunit (Gαs) and β2-adrenergic receptor (β2AR) in which miRFP670nano or miRFP670 were inserted between the helical and GTPase domains of Gαs and into intracellular loop 3 of β2AR27,28.

Either [32P] 5'-end-labeled (∼0.5 nM, large RNAs) or internally labeled (SL and B5, ∼0.1 nM, using the bI3 conditions) RNAs were folded in their respective buffers and incubated with Mrs1 for 30 min at 37°C before filtering.

Nanoparticles composed of poly(lactic-co-glycolic acid), with polyethylene glycol coatings to resist bioadhesion, were internally labeled with caged rhodamine to make the particles photoactivatable.

The 5S rDNA probe was a 5S LT1 PCR fragment internally labeled with α[32P]-dCTP by random priming.

Substrates were internally labeled by 3'-filling to enable assays of 5'-3' exonucleolytic digestion initiating opposite the structured end (Fig. 5A).

Biodistribution data obtained in DBA/2J mice with BPD-MA (monoacid ring A analogue) which had been tritiated or internally labelled with 14C showed that both labelled materials acted in an essentially identical manner during the period of study.

RNA probes internally labelled with 32P were generated using the T7 Riboprobe in vitro transcription kit (Promega).

For internally labelled oligos, a 35-mer oligo (5′-TGTCTGACCTTGTTTTTGGGACGTCTACTCATCTC-3′) was P labelled at the 5′ end with T4 polynucleotide kinase.

Two complementary oligonucleotides (0.5 nM each) were internally labelled with Cy3 and Cy5 dyes and were separately pre-incubated with RAD52 or RAD52Y104pCMF.

Radioactive probes that were internally labelled were made with the Mirvana probe construction kit (Ambion) with the primers listed in Supplementary Table 1.