In vitro, its derived peptide pep04 killed only virulent fungi, and confocal microscopy showed that it was internalized only by the virulent isolate.
The data demonstrates that ligand-QD complexes were exclusively localized at the plasma membrane and internalized only after subsequent incubation at 37°C.
This recombinant toxin binds to and activates both EGFR and ErbB-4, but is efficiently internalized only upon binding to EGFR.
This result is consistent with our findings that VEGF121/rGel is cytotoxic only to VEGFR-2-expressing endothelial cells [ 10] and is internalized only into endothelial cells that express VEGFR-2 but not VEGFR-1 (this study).
Comorbidity was related almost linearly with mental age and presentation (internalized only or mixed presentation), which supports the numerous studies reporting the emergence of conduct disorders before anxiety disorders [ 4].
A low uptake efficiency was, for example, found by Höcherl et al., concluding that HeLa cells internalized only 10−3% of the initially added negatively charged 160 nm poly methyl methacrylate) particles after 2 h based on flow cytometry and CLSM characterization [ 55].
For example, if a cell internalizes greater than 5 particles, cell death is induced within fifteen hours whereas if a cell internalizes only 1 silica particle, it will take double the length of time to induce death (Fig. 7, n = 37 cells).
Calvarial osteoblasts and macrophages are able to uptake the soluble fraction of bisphosphonates and internalize only the pool of bisphosphonates which naturally resorbs [ 43, 44].
This group suggested that the difference in accumulation is because normal cells form tight intra-connected colonies and therefore, NPs can be internalized mostly only at the edge of a growing colony, while in malignant cells, the cell cell and the cell matrix connection is disturbed and therefore, NPs can be internalized into any cell on the tissue culture plate (Gal et al. 2015).
In vitro studies indicated that PEG-coated cationic liposomes were internalized not only by tumor cells but also by endothelial cells, and consequently its l-OHP formulation displayed higher cytotoxicity towards both cell types as compared with l-OHP containing PEG-coated neutral liposomes.
