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Some of the internalized material is delivered to the degradative vacuole after passage through early and late endosomes.
Eukaryotic cell processing of internalized material classically involves transport from early to late endosomes, and then to lysosomes where degradation typically occurs.
Early endosomes bud from sorting endosomes and transport internalized material to lysosomes or recycling endosomes.
Many of these were complex with lipid whorls and internalized material, including mitochondria, peroxisomes and rough endoplasmic reticulum, in various stages of digestion.
Our observations of elevated endolysosome pH could help to explain alterations in the digestive capability of endolysosomes as evidenced by decreased specific activities of three different endolysosome enzymes, which results in increased accumulation of internalized material thus altering the structure and size of endolysosomes.
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DOI: http://dx.doi.org/10.7554/eLife.03648.002 Clathrin-mediated endocytosis is a conserved and ubiquitous process for internalizing material from the cell surface.
CD19 and CD20 positive APC were excluded from the analysis as they had likely formed conjugates with B cells but had not necessarily internalized cellular material.
Endocytosis is an active process whereby cells internalize extracellular material through cytoplasmic membranes.
70, 71 In general, only certain types of cells can internalize particulate material with size above 200 nm.
Phagocytosis is the process by which macrophage, neutrophils and dendritic cells internalize particulate material over 0.5 μm in diameter.
Finally, DCs cocultured with netting neutrophils internalize NET material and, when transferred to rodent models, induce a vasculitis-like syndrome [ 51].
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