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We detected recombinant proteins, which represent bound and internalized components, by western blot using the anti-ES antibody.
Mechanistically, this involves formation of autophagosomes that are fused with lysosomes to facilitate digestion of the internalized components by lysosomal proteases.
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While the significance of this bacterial internalization in the pathogenesis of otitis media is not yet known, it is possible that such internalized bacterial components can also induce β-defensin up-regulation.
We show that VT680 diffuses into leukocytes within minutes, covalently binds to cellular components, remains internalized for days in vitro and in vivo, and does not transfer fluorescence to adjacent cells.
These pores are essential for delivery of the enzymatic A components of the internalized toxins from endosomes into the cytosol of target cells.
Salmonella isolates from internalized condition showed considerable internalization potential.
However, embryos depleted of each component exhibited a substantial delay in the degradation of internalized GFP CAV-1, which remained on internal membranes well beyond the two cell staGFP CAV-11C).
In addition, arrestins recruit components of the endocytic machinery, and internalized MORs are subsequently resensitized by recycling back to the plasma membrane (von Zastrow, 2010).
Assuming a membrane orientation similar to the plasma membrane flippase (i.e. such that phospholipid is transferred to the cytosolic leaflet), one possible function for ATP8a1 would be to "recondition" surfactant internalized via the recycling pathway by removing undesirable phospholipid components such as lysoPC from the LB for transport back to the ER.
As such, we suspected that this additional internalized mass within AH vaults might represent additional cellular components that coassociated within the AH vault lumen via interactions with the NS5A1-31 amphipathic α-helices.
Interestingly, the analysis of the fMLP-induced internalization of the receptors in cells depleted of any of the components of the GIT complexes analysed showed a clear inhibition of the perinuclear accumulation of the internalized FPR.
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