Similarly, only monocytes internalized large amounts of dextran (Fig 1E), a marker of fluid-phase endocytosis, and confocal microscopy demonstrated intracellular co-localization of fluorescently labelled N-BP with dextran (Fig 1F).
Indeed, in cultures of PBMCs, only CD14+ monocytes internalized large amounts of fluorescently labelled N-BP, while little or no uptake by other cell types, including CD3+ lymphocytes, could be detected at the concentrations and treatment duration used (Fig 1B-D).
Overall, the results suggested that dendritic cells could internalize a large amount of SWNTs without compromising cellular integrity.
Large amounts of HAMLET were internalized by the tumor cells and co-localization of HAMLET and α-actinin-4 was detected at the cell periphery already after 15 minutes (3D z-stack images in Fig. 2B, Movie S1, for corresponding light images see Fig. S2B).
This endocytic pathway is capable of internalizing Cholera toxin, GPI-linked proteins, and large amounts of extracellular fluid, and it corresponds (at least in part) to the CLIC/GEEC endocytic pathway.
Ms. Charnock: Large amounts of personal mentoring.
