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This can be related, in accordance with (23) and (26), to a corresponding decrease in the concentration of internalized toxin T i and a consequent increase in the toxin internalization time (since it takes longer to achieve a give level of T i ).
We found that PrPC was present in DRMs and that it remained associated with them during the entire internalization time, suggesting that it does not exit "raft domains" to be internalized via the clathrin-coated pits.
The peak internalization time (4 h) was again confirmed by the images obtained from cLSM analysis.
We previously showed that AF594 labeling does not disrupt HPV particle binding to the extracellular matrix (ECM) or cell plasma membrane, antibody-mediated neutralization, internalization time, or infectivity [11].
The relationship between the internalization time τ i and Ψ described in (27) is shown in Figure 8. Ψ was determined from simulated toxin internalization time courses as the time to internalize 5 · 10−14 M ricin.
To get better cellular images, the sensor concentration of 0.1 mg mL 1 and an internalization time of 16 hours were usually used for cell staining.
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We note that in contrary to B, the total level of receptor does not increase in the region of cooperation (around point I in Figure 3A), due to the different internalization times of the unbound and bound receptor.
Transcriptomic analysis using an oligoarray covering the whole S. aureus genome was performed at two post-internalization times and compared to gene expression of non-internalized bacteria.
Using such multiplex assays and CdSe ZnS core-shell quantum dots, Kriete et al. were able to quantify rapid changes in epidermal growth factor receptor internalization over time [84].
This review overviews the area of cyclometalated iridium III) complexes in biology, with an emphasis on comparing the various conditions that these compounds have been assessed for their biological potential, such as the specific tested cells lines, concentration of internalization, incubation time, and mechanism of cellular entrance.
The data were expressed as percent internalization over time [20].
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