Exact(2)
We would like to thank Arbi Nazarian, Ph.D. for his assistance in setting up the internalization protocol.
The GLUT4 myc internalization protocol was adapted from previously established protocols (Ishikura et al., 2010).
Similar(58)
In our laboratory, we have recently developed a protocol for internalization by electroporation (Crawford et al. 2013; Fig. 1a).
Recently, we developed an electroporation-based protocol for internalization, which allows biomolecules labeled with organic fluorophores to be introduced at high efficiency into live E. coli (Crawford et al. in Biophys J 105 (11):2013–2013, 2013).
When tagged D2 receptors were expressed in locus coeruleus neurons, a desensitizing protocol induced significant internalization.
If a cell filtration step is added to the protocol, the non-internalized spots are removed and cell-internalized fluorescence is revealed, corresponding to the internalized monomeric fraction of Pol I. Using the above guidelines, we optimized the internalization of the 10-kDa omega subunit of bacterial RNA polymerase, labeled with Cy3b.
VZV internalization was assayed by modifying a previous protocol used to determine internalization of Kaposi's sarcoma-associated herpesvirus [66].
We illustrate the usability of the optimized protocol by demonstrating high-efficiency internalization of a 10-kDa protein, the ω subunit of RNA polymerase.
Salmonella isolates from internalized condition showed considerable internalization potential.
To induce NMDAR internalization pharmacologically, we used 2 different treatment protocols.
Phage display biopanning in Round 1 is performed using established protocols with cell binding and internalization as the selection criteria.
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