The delayed internalization might point at a different internalization and trafficking route to EEs of monomeric receptors as compared to ubiquitinated EGF-EGFR complexes (Sigismund et al., 2013).
"There's usually an internalization period similar to just enjoying a record," says Robinson.
Both cell lines lacked the capacity to significantly internalize TF‐LPA above baseline levels (Fig 1H), strongly arguing against an important role for phosphorylation explaining the loss of function of PRG‐1R346T in TF‐LPA internalization, however, pointing to the need of S347 at this position.
To examine its effect on tubule fission from early endosomes, we added dynasore to cells at 5 min post-internalization, a time point when transferrin was mainly localized in the early endosome.
TR-Tf internalization at each time point was quantified as previously described [53].
We did not detect obvious internalization events at time point of 10 min after the immune recognition of B1-8-BCR and NP-TGT.
26 This decrease was also evident by 4 hours after treatment (Fig 5C, D), consistent with immunostaining data demonstrating significant antibody-mediated receptor internalization at this time point.
The wild-type AP1 strain was found to be slightly more resistant to internalization at early time points (Fig. 3B), but after one hour no difference could be seen between the two bacterial strains.
Author reply: With respect to the specific point (that saltatory internalization might be deleterious to the host, Paragraph 22), we are here examining an issue also raised by other authors (note reference), and thus do not feel we need to reply to the specific point in detail.
The degree of GluA1 endocytosis was determined by analysing the first 1 3 min after NMDA stimulation (max decrease in signal) and the rate of GluA1 recycling was determined by fitting a linear curve to the time after max internalization and calculating the time point at which 50% of fluorescence recovered.
