Treatment with 20 μM of LY294002 for 90 min caused BCRP internalization from the cell surface.
Receptor internalization from the cell surface occurs through several mechanisms.
Therefore, if PLP travels by transcytosis, internalization from this domain will occur.
One possibility is C-cadherin internalization from the cell surface via endocytosis, as suggested for FLRT3 [4].
The BiFC signal exhibited copper-dependent internalization from the cell surface, with the progressive appearance of vesicular fluorescence.
The advancing boundary moves into the PM region largely unaffected by the induced internalization from within the contact area.
Representative confocal images from animals that received IPLT formalin show increasing NK1-R internalization from L1 through L5.
Using this method, we determined that the Ctr4 and Ctr5 proteins at the plasma membrane underwent internalization from the cell surface after copper addition.
Therefore, if PLP travels by transcytosis, after its transport from the Golgi to the cell body plasma membrane, internalization from this domain might take place.
Note that VEGF levels are depleted to ∼0.988 pM at the tip cell due to internalization from the 104 VEGFR2/cell along the sprout length (Fig. 2C).
The model therefore did not incorporate any positive feedback from receptor engagement that could work against the negative feedback of the accelerated receptor internalization from the interface.
