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Therefore, internalization data (Table 2) are normalized to the uptake of ([125I]I-BA KuE, that was always assayed in parallel as an external reference.
Description of data: Statistical analysis of the nanozeolite internalization data for the different types of nanozeolites in the same time point and for the same type nanozeolite in different time points.
Based on unlimited precursor availability, [68Ga]HA-DOTATATE therefore represents a useful alternative to other currently used 68Ga-labeled somatostatin analogs and, as suggested by affinity and internalization data, may have potential for targeted radiotherapy using, e.g., 177Lu.
To avoid the influence of inter-experimental variations in cell count and cell viability on absolute internalization data, all internalization experiments were performed as dual-tracer studies using [125I]TOC as an internal reference.
Both the complexes were cytotoxic in dark and exhibit significant enhancement of cytotoxicity upon photo-exposure against HeLa and HepG2 cancer cells giving IC50 values ranging from 8 to 12 μM for 1 and 2. The cellular internalization data showed cytosolic and nuclear localization of the complexes in the HeLa cells.
To better assess the phagocytic ability, we combined interaction and internalization data.
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With respect to the MBCD effect on TTR internalization the data show no significant changes on internalized V122I TTR at 4°C (5.9±1.7 versus 6.4±4.2% without versus with MBCD, respectively) and a slight decrease at 37°C (16.1±0.2 versus 10.8±3.3% without versus with MBCD).
In NECA-induced internalization assays, data were normalized to basal (in the absence of NECA) and maximal internalization, and in assays using HEMADO, data were normalized to basal (in the absence of agonist) and a 10 μM NECA control which was included in each separate experiment.
With respect to the dimension of Internalization, the data show that at the outset relations between the professionals from the various organizations were marked by mistrust and prejudice.
Only cells with similar IR-B-SYFP expression levels (estimated by the VFP intensity signal) were considered since high expression levels of IR affect internalization rates (data not shown).
Internalization and priming data were analyzed by pairwise comparison by Student t test for independent and dependent samples, respectively.
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