As an internal control for MHC-I internalization, these ΔY-derived DCs efficiently internalized endogenously-expressed wild-type Kk molecules (33% internalization at 30 minutes of chase), albeit not as rapidly as observed for KbWT DCs (Figure 2C).
Our results show that this nanoconstruct is highly pH-sensitive in a mildly acidic pH, exhibiting high binding and internalization at pH 6.8 and below, but low binding and internalization at pH > 7.
Enterocyte preincubation with TNF-α alone, and with both cytokines, was associated with decreased S. aureus internalization, at P < 0.05 and P < 0.01, respectively.
The potency of CGRP at stimulating cAMP production was reduced at Leu41Ala, Gln45Ala, Cys48Ala and Tyr49Ala; furthermore, CGRP-induced receptor internalization at all of these receptors was also impaired.
It effectively silenced GFP expression in 40 70% GFP-expressed HEK293 cells and it was observed that higher N/P ratio resulted in more effective silence which was likely due to better cell internalization at higher N/P ratio.
The probes QE and bivQ showed comparable binding and internalization at the physiologically relevant temperature of 37°C only to CCK2R-transfected A431 cells (Figure 2a,b).
After correction for acid-resistant surface binding, the internalization at 37°C was calculated as the percentage of the total amount of radioactivity specifically bound (internalized and surface-bound) to the cells.
TR-Tf internalization at each time point was quantified as previously described [53].
To quantify NK1-R internalization, we first standardized characterization of NK1-R expression and internalization at high magnification (63×).
PTRF/Cavin silencing affected significantly IGF-IR plasma membrane recovery at 30 min and reduced IGF-IR internalization at 5 min without reaching a statistical significance (Fig.3A).
However, binding of the Gd3+ proteins to the cells triggered receptor mediated internalization at 37°C as demonstrated by the staining of the intracellular ProCA1-affi.
