Also, the introduction of a second, constitutively expressed luciferase in the same cell can provide an internal viability control to correct the analytical signal, improving assay reliability and robustness.
Besides, this methodology can be used, for instance, to simultaneously compare expression of stage-specific gene products, measure distinct cellular pathways, or evaluate the activity of an inducible or treatment-responsive promoter as compared to a constitutive internal viability control, respectively driving the expression of the two luciferases in the same parasite.
These finding suggest that AMPs can inhibit the H+ pumping F1F0-ATPase of mycobacterial plasma membrane that potentially interferes the internal pH and viability of mycobacteria.
This was anticipated to be an optimal host due to its high internal salt concentration, viability at low temperatures, completely sequenced genome, lack of endogenous β-galactosidase, and many available microbiological and molecular genetic tools [ 12, 24, 25, 38].
The results show that with a linear heat density greater than 1.5 MWh/m, there could be viability with internal rates of return higher than 7.4 and 9.8%, and payback period below 13 and 10 years, for solar and biomass systems respectively.
Four years into the evaluation, the evidence indicates EPHS has responded effectively to external and internal changes to ensure viability.
To our knowledge, however, the negative relationship between light exposure on the gills (or other internal organs) and organismal viability has not been elucidated to date.
NHS foundation trusts, those organisations defined as 'high-performing' hospitals and are run as not-for-profit corporations, have an additional layer of regulatory oversight provided by the economic regulator - Monitor - which imposes authorisation standards in relation to internal governance and financial viability.
A recent study attributed enhanced cell viability to the internal structure of the scaffold rather than to the type of coating material used [15].
Airbus has been developing its autonomous vertical take-off and landing (VTOL) concept through Project Vahana, an internal project designed to test viability and refine a prototype for urban air transport.
Scaffolds with porous internal architecture retained higher cell viability and activity than solid scaffolds, likely due to increased oxygen and nutrients exchange into the interior of the scaffolds.
