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Because a puncture in their fur could mean death, scientists use internal tags on otters instead.
Accordingly, we only used internal tags to further explore the physiological dynamics of Cse4.
However, we found no such bias for internal tags that disrupt essential proteins: 14 tags inserted within annotated domains vs 12 inserted within unannotated sequences (χ, p = 0.7).
The other internal tags, one of which is highly represented (tag 189, see additional file 1), may be due to either alternative polyadenylation events or priming at internal polyA stretches during cDNA synthesis, as hypothesized by others [ 24].
Manual checking of these tags within their mRNAs, showed that indeed >98% of them represent the start of the poly(A) stretch, and do thus not represent internal tags.
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To evaluate miRFP670nano as an internal tag, we constructed internally labeled G protein α-subunit (Gαs) and β2-adrenergic receptor (β2AR) in which miRFP670nano or miRFP670 were inserted between the helical and GTPase domains of Gαs and into intracellular loop 3 of β2AR27,28.
This means your individual behaviour can carry a social tag independently of the internal tag you may assign it.
Such proteins can be labeled with FP inserted in a middle of the sequence as an internal tag.
In contrast, two-domain BphP-derived NIR FPs, which have the figure-of-eight knot in their structure and distant termini, are not suitable for internal tagging.
Microsoft Microsoft is experimenting with an internal tagging system, a javascript bookmarklet that can only be used to save pages on Microsoft.com.
The haplotype blocks were defined using the solid spine of LD approach, using Haploview's internal tagging program.
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