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Internal standardization (standard addition with spiked samples) was employed.
Also additives (in liquid or solid form) can be introduced for internal standardization, quantification (e.g., for standard addition or isotope dilution purposes), or to affect laser sample interaction (i.e., increased energy absorption at the applied laser wavelength).
In targeted analysis, SIL reference standards can be used for internal standardization of peak intensities in trace analysis by use of LC HRMS or LC–(HR MS MS [ 27, 28], making this the most common use of SIL.
29Si was used for internal standardization and data were calibrated against two standard reference materials (NIST SRM 610, 612) and Corning B, C and D. The elemental compositions were then calculated according to Gratuze [38].
Quantitation was achieved by internal standardization using stable isotope-labeled amino acids in cell culture (SILAC).
Intermediate precision and matrix effects remained acceptable for most analytes thanks to efficient internal standardization.
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Positive reactivity for each reaction was determined by computation of the difference in Ct values between the sequence-specific and the internal-standardization reactions and comparison to empirically determined cut-offs.
The integration of a pooled internal standard [ 26] enables a standardization of all gels included in the analysis, lowering the gel to gel differences in the data analysis, which is the main technical variation in human skeletal muscle proteomics [ 27].
Internal standards serve to allow production control and product standardization, and to adjust production cost to various product requirements and different quality levels.
This is demonstrated in a scatter plot of the estimates from each simulation in Fig. 3 (internal age standardization) and Fig. 4 (external age standardization).
Second, although our cytometric procedure performs robustly, accurate internal methodological standardization is strongly recommended in order to avoid misclassification especially of borderline cases.
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