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100 μL of standards was mixed with 100 μL of the internal standard solution and used as reference standards.
One milliliter of this solution is added to 5 milliliters of an internal standard solution, and then diluted with more solvent to a final volume of 10 milliliters.
Remove the plate from the incubator and add the internal standard solution (100 μl) to each well and methanol (700 μl).
50 μL of rat plasma was mixed with 100 μL of an internal standard solution [lysoPA(17:0.1 0.1 nmol mL−1 in methanol], vortexed and added to an additional 350 μL of methanol.
The concentration of the working internal standard solution of 4-hydroxydiclofenac was 0.0045 mg/mL.
Samples were prepared volumetrically using an internal standard solution in 2% nitric acid.
A 5.0-μL aliquot of the internal standard solution was added.
An internal standard solution (3.58 mg/mL) was prepared by resolving styralyl propionate in MTBE.
50 μL of working internal standard solution of allopurinol was added to each sample, standard and quality control.
100 μL of each sample and 100 μL of the internal standard solution were pipetted into a vial.
Calibration level 1 consists of 10 µL SD 1 and SD 2 plus 100 µL internal standard solution.
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