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I.S.1: internal standard hexadecane, I.S.2 internal standard tridecanoic acid and *instrument contamination.
Figure 11 Chromatograms obtained after extraction with CH 2 Cl 2 /MeOH of A) Sample 1, B) Sample 2, C) Sample 3 and D) Sample 4. I.S.1: internal standard hexadecane, I.S.2: internal standard tridecanoic acid and *instrument contamination.
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S.I.1 internal standard of injection (hexadecane); S.I.2 internal standard of derivatisation.
Hexane with an n-hexadecane internal standard was co-distilled with the samples and acted as a solvent trap for volatiles.
n-Hexadecane (internal standard, IS1), tridecanoic acid, (internal standard, IS2), hydrochloric acid (HCl), potassium hydroxide (KOH) and N,O-bis trimethyl)silyltrifluoro-acetamide (BSTFA) coN,O-bis trimethylthylchlorosilane, were purchased from Sigma–Aldrich (Milan, Italy).
A 5 μl top layer was mixed with 995 μl n-hexane containing 1 mM n-hexadecane (internal standard) to prepare samples.
To lower solvent evaporation as much as possible, the high-boiling N, N-dimethylacetamide (DMA) was used as a solvent and n-hexadecane as an internal standard for quantitative GC analysis.
Dodecane or n-hexadecane were added before extraction as internal standard respectively.
After adding 10 μL of n-hexadecane solution (80 μg g−1) as an internal standard for the injection, 2 μL of the solution were analyzed by GC/MS.
I.S. = internal standard (d-ribose).
Tridecane standard was used as internal standard.
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