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The tumor revealed peripheral early enhancement and delayed internal staining in dynamic computed tomography images and marked high intensity in diffusion-weighted magnetic resonance imaging scans.
Imaging findings of our case such as peritumoral early enhancement, delayed internal staining, and the absence of bile duct dilatation were similar.
Internal staining for Oct4 was performed using the Cytoperm/Cytofix Kit (BD).
Cell pellets of each PCa cell line (RWPE, LNCaP, 22Rv1, DU145 and PC3) were spotted on each array and served as internal staining controls.
Furthermore, at the permissive temperature 80% of wild type cells and 63% of the TS-mutant cells show a distinctive perinuclear internal staining presumably representing multiple endosomal compartments containing FITC-transferrin [43] as has been observed before [44].
On day 7 post-infection (at the peak of response), the CD62L negative and CD62L positive B8R20 27 pentamer+ CD8 gated cells were examined for the expression of CCR7, IL-7R, IL-15R, IL-18R (by external staining) and granzyme B (by internal staining).
Similar(43)
"Internal stains are deadly to the deal," Dr. Campbell said.
This prominent internal stain, probably representing accumulated endosomes [43], shows major co-localization with FITC-transferrin (figure 6).
Uninterpretable results due to poor internal control staining were more common in liver metastases than in primary CRC.
One cytology stain that failed internal control (no staining at all: no p27 staining in normal cells or no Galectin-3 staining in inflammatory cells) was considered invalidate and removed from the statistical analysis.
By contrast, upon knock-down of Cav-1, L929 cells exhibited increased internal 22L PrPSc staining compared to cells transfected with non-silencing siRNA (Fig. 3e).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com