GS ROX 500 internal size standard (Applied Biosystems) was included in all assays.
When the difference in internal size became too large, the surface energy effect mitigated.
(SBA spokesman Mike Stamler responded that the SBA has told banks they "could use their own internal size standards" for non-SBA loans).
The fragment size was estimated by comparison with the internal size standard GS-LIZ500.
An internal size standard (ET 400-R, GE Healthcare) was run in every sample.
Finally, the internal size standard LIZ500 was added to all samples prior to loading on an automated sequencer.
All reactions incorporated the GeneScan 350 ROX internal size standard (Applied Biosystems Inc ., and were run for 35 cycles in a Mastercycler gradient thermal cycler (Eppendorf).
The fluorescently labeled 5' terminal-restriction fragments were sized against the Genescan ROX-500 internal size standard using GeneMapper v3.7 (Applied Biosystems).
The Genescan 350/500 Tamra was used as internal size standard within the fragment analysis.
Allele sizes were determined with the internal size standard GeneScan-400HD ROX (Applied Biosystems, Madrid, Spain).
Additionally, internal size standard GENESCAN™, TAMRA (Perkin-Elmer, Germany) was added to the mixture.
