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Partial digestion was minimized by an internal restriction site and a control sample of digestible homozygous Val/Val.
In the construction of C-terminally truncated topoIIβ, multiple internal restriction sites in the topoIIβ sequence meant that a complex cloning procedure was necessary.
Full length coding sequences were inserted by T4 DNA ligation of N-terminal β2-subunit isoform fragments cut by BamHI (pCR2.1 restriction site) and HindIII (internal restriction site contained in all β2-subunit isoforms) and of the C-terminal fragment cut by HindIII and NotI (pCR2.1 restriction site).
The HELP cocktail strategy required an extra step to filter contaminating fragments that did not harbour an internal restriction site.
An HindIII internal restriction site was also observed, plus partial sequences probably caused by recombination, so that only 35% (7/20) of scFv sequences were full-length.
The minisatellites were named according to their order of detection and the satellites according to conserved internal restriction sites (Table 1).
Similar(45)
Most Palestinians have been unable to reach Israel since the current round of violence began three years ago, but the internal restrictions make normal life virtually impossible.
"Men and women of the F.B.I. in the field are frustrated because many of our own internal restrictions have hampered our ability to fight terrorism," Mr. Ashcroft said to reporters.
In Russia, internal restrictions on foreign travel melted away with the Soviet collapse, and the freedom to go abroad, especially to the 27 countries of the European Union, is cherished.
We passed some laws and the FBI placed some internal restrictions on the way it would operate that most people think were pretty much complied with.
To address the concern about internal minorities, Kymlicka distinguishes between two kinds of group rights: "external protections" are rights that a minority group claims against non-members in order to reduce its vulnerability to the economic and political power of the larger society, whereas "internal restrictions" are rights that a minority group claims against its own members.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com