The atomic model of the protein shows a homodimeric association of two polypeptide chains, each containing an internal repeat of a ferredoxin-like βαββαβ fold, as seen in the ACT and RAM-domains.
An NI3C C-cap mutant carrying five additional mutations in the interface to the preceding repeat, previously designed by using the consensus sequence as a guide, showed a rigid-body movement of the C-cap towards the internal repeat.
To further understand GpTx-1 binding to NaV1.7, we have mapped the binding site to transmembrane segments 1 4 of the second pseudosubunit internal repeat (commonly referred to as Site 4) using NaV1.5/NaV1.7 chimeric protein constructs.
And Idina Menzel's (a.k.a. Adele Dazeem's) rousing Oscars performance of the hit "Frozen" tune on Sunday only fueled the internal repeat loop.
We show for the first time a role for a gammaherpesviral internal repeat in the pathogenesis.
We used infection of laboratory mice with murine gammaherpesvirus 68 (MHV-68) to explore the biological role of the 40 bp internal repeat of MHV-68.
Here, we investigated the biological role of the 40 bp internal repeat by infecting mice with mutant viruses partially or completely lacking this repeat.
A deletion mutant (Delta 40 bp mutant), completely lacking the 40 bp internal repeat of MHV-68, was constructed by ET cloning [26], [27] as described [25].
To determine the role of the 40 bp internal repeat in pathogenesis, three mutants were constructed using MHV-68 cloned as bacterial artificial chromosome (BAC) (Fig. 1A).
As described previously [25], BAC clones of MHV-68 may loose a certain number of the 40 bp internal repeat units due to recombination between the direct repeats.
The 40 bp internal repeat appears to be largely dispensable for lytic replication both in vitro and in vivo, however, it plays a role during amplification of latency.
