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The RT-PCR included pan lyssavirus-primers (N gene) and internal PCR control primers for ribosomal RNA.
After sequencing, this was found to be a unidirectional PCR product from the forward primer, terminating outside the deleted region and was also used as an additional internal PCR control.
In addition to these human nuclear targets, the assay includes an internal PCR control target sequence to allow for an assessment of PCR inhibition.
An internal PCR control (IPC) is incorporated into the kit to reliably and consistently identify PCR inhibitors that may interfere with downstream processes, such as STR PCR.
Telomerase activity was calculated according to the following formula: TPG = [(X−X0)/C]∶[(r−r0)/Cr*100], where TPG is the total product generated, X signifies each sample, C represents the 36 bp internal PCR control, r is the TSR8 quantification control.
GAPDH gene expression was used as an internal PCR control.
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The assay incorporates an internal PCR amplification control (IC) template system enabling PCR amplification conditions to be assessed in each reaction.
Actin was used as the internal PCR positive control.
Actin was used as an internal PCR positive control.
B. suis primers were used as an internal negative PCR control.
This PCR method presents both GSTM1- and GSTT1-specific primer pairs in the same amplification mixture and includes a third primer pair for β-globin as an internal positive PCR control.
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