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The internal mutation of a genre reflects the changing tastes and mores of the public.
Two hypotheses have been proposed, (i) internal mutation theory and (ii) circulating high virulent-low virulent theory.
Our data provides strong support for the internal mutation hypothesis, as the mutations are unique to individual cats.
The so-called internal mutation hypothesis, which postulates that viruses transition from avirulent to virulent via certain mutations leading to FIP pathogenesis, is generally believed in veterinary medicine [ 10].
A contrasting view to the internal mutation hypothesis to explain the genesis of FIP outbreaks is that there is a circulating FCOV other than FECV that is specific for FIP (22 ).
For example, there are two probe sets in the array representing rpoS (probe set IDs: 1761030_s_at and 1767783_s_at) because the rpoS sequence in the strain CFT073 harbors an internal mutation that results in two truncated genes, c3306 (519 bp probing to 3' end of rpoS) and c3307 (435 bp probing to 5' end of rpoS).
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Changing multiple regions involves large internal mutations that often lead to receptor instability.
The human body is constantly being attacked by internal mutations as well as external pathogens.
Thus, transition to FIP may occur through changes in viral populations in a feline host rather than internal mutations.
For all the members of these four gene families, no internal mutations in the regions bearing the putative TAC sites have been found.
The egfp gene harboring internal mutations (indicated by a red box) is repaired through ZFN-mediated cleavage and HR using the reverse-transcribed vector as a recombination donor.
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