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A very sensitive high-throughput screening method based on capillary electrophoresis has been developed (Cross et al., 2007) using Endonucleolytic Mutation Analysis by Internal Labelling (EMAIL) to greatly improve the effectiveness of this new reverse genetics approach to crop improvement.
Washing the cells after Laurdan staining also helped to prevent internal labelling, removing excess, unincorporated Laurdan and enabled imaging for sixty minutes before internal structures started to show labelling, which was more than sufficient for our experimental purposes.
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An additional feature to consider when labeling target material is whether to use end-labeling or internal labeling strategies.
Here we describe a strategy for determining the position of internal protein domains within EM maps using a recombinant one-step labeling approach named Efficient Mapping by Internal Labeling (EMIL).
"A lot of times NLP companies will have fairly 'comprehensive' knowledge graphs where you do internal labeling, but most of the data comes from the product catalog," Wu, the CEO, said.
Internal labeling of BrdU-containing templates with [α-32P]dCTP is described below.
A label is allowed to appear once as a leaf and once as an internal label.
Apart from the internal labels, this tree is identical to the original one.
We found that surface and internal labeling by immunofluorescence or GIRO yielded similar distributions for surface and internal channels.
The transferrin-conjugated QDs also showed clear internal labeling in the fungi Schizosacharomyces pombe and Penicillium chrysogenum.
Internal labeling of FAP-BKα was also significantly decreased, but this decrease was to a lesser extent than surface labeling (15.3% decrease).
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