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The original Edison bulb was pear-shaped, built around an internal filament.
We then added sAB-4 to the flow cell, which triggered the formation of multiple internal filament breaks (Fig. 4b), providing direct evidence that sAB severs the actin filaments.
Whole cell recordings were obtained using patch electrodes pulled from borosilicate pipettes (1.2 mm outer diameter, 0.95 mm inner diameter, with internal filament) using a Narishige PP-830 vertical puller.
Whole-cell recordings were obtained using 8 15 MΩ patch electrodes pulled from borosilicate glass (1.2 mm O.D., 0.95 mm I.D., with internal filament, World Precision Instruments, Sarasota, FL) on a PP-830 micropipette puller (Narishige USA, East Meadow, NY).
Pipettes were fabricated from borosilicate glass with an internal filament using a Sutter P-97 puller and had a resistance of about 22 MΩs when filled with 3 M KCl.
The internal filament (ranging from 3.6 to 4.9 mm) was ∼75 % the size of the external one (ranging from 6.6 to 7.3 mm), with SM presenting the shortest filament and TMS the longest (Table 1).
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One extension was at least 585 nm long (continued to the edge of the reconstructed area) and contained two internal filaments while the other was much shorter and contained a "Y" shaped inner vesicle protruding into the extension.
In order to imitate boB in synthetic implants designed to bridge nerve lesions, we developed resorbable, semipermeable nerve guide conduits with microstructured internal polymer filaments.
The cell is modeled as a granular medium, using rigid spheres (grains) corresponding to intracellular cross-linking proteins and distant mechanical interactions to reproduce the cytoskeleton filament internal forces.
While producing some artifacts, these methods have been profoundly successful, filling our textbooks with information about the structure and positions of cell walls, internal membranes, cytoskeletal filaments, and even large cytoplasmic particles like ribosomes.
Differences in the filaments' internal architecture result in a wide variation of their bending rigidity.
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