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As genes responsible for proton transport were identified to be differentially expressed in P. temperata, we evaluated impact of external pH conditions and internal expression of proton transport genes on bacterial growth in vitro, as well as influence of external pH conditions to bacterial persistence in the nematode.
There is a fluency to my internal expression to God.
The housekeeping gene of H. longicornis β-actin was used as an internal expression control.
Bistable and multistable (and hence discrete) hysteretic switches, enabling cells to adopt multiple internal expression states in response to an external input have a pivotal impact on biological systems, ranging from cell-fate decisions to cell-cycle control [26].
Internal expression controls were done by using β-actin (Sigma-Aldrich).
Apart from an internal expression language called 'GREL', Jython and Closure may be used for coding.
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UBQ10 was used as the internal control; expression in mock-treated Col-0 seedlings was used as the calibrator, with the expression level set to one.
Results were expressed as density of transgelin 2 in relation to β-actin, an internal control, expression within the same sample.
The results of internal gene expression analysis of three independent experimental models of mitochondria and insulin signaling perturbations further support the connecting roles of these genes.
The ΔU3-HIV[MLV] vector carried a −418 to −40 U3 deletion and was obtained by inserting an internal ΔLNGFR expression cassette driven by the full MLV LTR into the pRRLsin-40.GFP vector [51].
The ΔU3-HIV[CMV] vector carried −418 to −18 deletion in the U3 region and an internal GFP expression cassette driven by the cytomegalovirus (CMV) immediate-early promoter, and was previously described as pRRLsin-18.pptCMV-GFPwpre [50].
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