Sentence examples for internal exogenous from inspiring English sources

An internal exogenous standard (cDNA synthesis with no reverse transcriptase) was also run separately for each cDNA mixture.

The assay comprises the amplification of two independent targets, MSC_1046 and MSC_0136, as well as an internal exogenous amplification control in a multiplex reaction.

Therefore we developed a novel TaqMan®-based real-time PCR assay comprising the amplification of two independent targets (MSC_0136 and MSC_1046) and an internal exogenous amplification control in a multiplex reaction and evaluated its diagnostic performance with clinical samples.

We show that the PESP was rapidly adopted because of the particular domestic context, plus several internal and exogenous factors.

Samples that were negative for the 16S rRNA gene of D. nodosus (n = 23) were amplified a second time with the TaqMan Exogenous Internal Positive Control Reagents (Applied Biosystems); this is an internal amplification control (IAC) for distinguishing true target negatives from PCR inhibition.

To address the problem of detecting inhibition in purified nucleic acids, an exogenous internal positive control (IPC) based on Taqman® chemistry was developed.

Both internal migration and exogenous immigration could provide needed supplements to the working-age population in many EU member states, particularly in highly technical sectors and in manual labor.

The purpose of this study was to design, validate, and optimize internally standardized real-time quantitative RT-PCR assays and to identify and avoid problems with assay reliability and examine the impact of an exogenous internal standard.

An exogenous internal reference RNA consisting of 5×107 copies of Tobacco Mosaic Virus (TMV) was introduced into each sample during total RNA preparation.

PCR assays were run on a ABI/Applied Biosystems 7900HT thermocycler using 384 well plates with Applied Biosystems exogenous internal positive control labeled with Vic in separate wells to test for the presence of PCR inhibitors.

A commercially available TaqMan exogenous Internal Positive Control (IPC Applied Biosystemss) was used to detect PCR inhibition.