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To further elucidate the interactions between these subunits, we generated two internal deletion mutants of GLP to delete either the TAD or the catalytic domain.
To delete the Pygo binding site (ΔHD1) in BCL9-2, an internal deletion of amino acids 238 265 (inclusive) was generated by PCR-mediated mutagenesis.
Differential staining also confirmed the location of the internal deletion of PrPSc106 at or near these densities.
HeLa cells contain MYPT1 in addition to a newly identified human variant 2 containing an internal deletion.
Three other internal deletion mutants of Nef, spanning amino acids 8 17 (NefΔ8–17), 25 35 (NefΔ25 35), and 57 66 (NefΔ57 66), were also studied.
The resulting protein, containing an internal deletion, would be partially functional but expected to lead to a milder, Becker muscular dystrophy-like phenotype.
External and internal deletion mutants for RB1CC1 (GenBank no.
Point mutations and internal deletion mutants were generated by standard PCR techniques and verified by sequencing.
The megaprimer method was used to generate internal deletion or point mutations [49].
An internal deletion was done (from amino acids 56 to 80 in NFAT5a), as described [20].
We generated 5' deletion constructs of pMARCKS-Luc(−1152) and an internal deletion construct of the region −700∼−1.
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