Sentence examples for internal control standards from inspiring English sources

Such normalization requires internal control standards with similar amplification efficiencies as the test target or external controls.

For the reporting of exact normal allele sizes (n=5 35), which are determined by PCR and subsequent capillary electrophoresis, internal control standards from sequence-verified samples should be included.

In addition, QuantumRNA™ 18S internal control standards (Ambion Inc., Huntingdon, UK), which produced a PCR product of 489 bp, were used at a ratio of 10 1 competitors to primers, in accordance with the manufacturer's instructions, to act as an internal loading control to normalize between samples during densitometry.

The expression ratios of the analyzed genes were calculated using an internal control standard curve of β-actin levels.

We have found, to great surprise, that widely-accepted internal control standard, GAPDH itself, was hypoxia-induced, when the cDNAs were normalized with respect to β-actin control.

Sample aliquots were labeled with two different fluorescent dyes (Cy3 and Cy5) and the internal control standard, actin, was stained with Cy2.

For internal control standard we used recombinant green fluorescent protein (GFP) (Vector Laboratories, USA), which was spiked in the plasma dilution mix (Olink Bioscience) and thereby added to all sample incubations.

The T. brucei dataset, obtained from the mzMatch.R project website, was chosen because it presents a set of metabolites identified with the aid of internal control standard compounds, being specially suited for performance evaluation.

In brief, 2 μL of a plasma sample was mixed with 2 μL of plasma dilution buffer (Olink Bioscience, Sweden) including 200 pM of GFP as internal control standard spike-in.

Quantitation of mRNA relative to the standard level of expression of the genes of interest was defined as a relative quantity (RQ) and was given by the following equation (Livak and Schnittgen, 2001):, where ΔΔ Ct==((Ct gene of interest− Ct internal control)sample−(Ct gene of interest− Ct internal control)standard).

Another concern that arises in regard to qPCR studies is that transcript expression is calculated relative to an internal control standard [ 62- 64]; which by definition is expressed at a consistent level regardless of experimental conditions or cell/tissue types under study.