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*Based on the Homo sapien gene sequences adopted from the National Center for Biotechnology Information GenBank Database [ 22]. a Housekeeping genes; b Internal control; # Normalization gene.
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Detailed information on handling of liver biopsy samples, protocols for RNA labeling, chip hybridization and signals, internal controls, normalization procedures, and analysis of gene expression were deposited in Gene Expression Omnibus [GEO GSE15235].
Differentially expressed genes, identified by microarray through global normalization, require validation of their expression patterns through Q-RT-PCR, which generally employees one internal control for normalization.
GAPDH was used as an internal control for normalization.
Most notably, the selection of an appropriate internal control for normalization is essential for expression data interpretation.
17S rRNA was used as an internal control for normalization.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as housekeeping internal control for normalization.
Water (NC) was included as a negative control and 17S rRNA was used as an internal control for normalization.
Actin was used as internal control for normalization (for primer, please see Additional file 1: Table S1).
Relative quantitation of the transcript was performed using co-amplified ribosomal 18S as an internal control for normalization.
Expression of 17S rRNA (accession number X00755) (Table 1) was used as an internal control for normalization of expression levels.
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