Sentence examples for internal control normalisation from inspiring English sources

GenomeStudio provides an internal control normalisation method for the 450K assay (Illumina, 2008), which is also used in MethyLumi (Davis et al, 2011) and Minfi (Hansen and Aryee, 2013); by default, Genomestudio uses the first sample in the array as the reference and allows the user to reselect the reference sample as needed if the original sample is nongenomic or of poor quality.

For each probe, the relative ratio was calculated using population normalisation and using the internal control probe normalisation.

GAPDH was used as an internal control for normalisation.

Real-time PCR reaction products were synthesised and quantified by SYBR Green I (iQ SYBR Green Supermix, Biorad) on an iQ5 Real-Time PCR Detection System (Biorad) using the ribosomal LP32 gene as an internal control for normalisation.

The luciferase reporter vectors (0.8  μg per well) (pNF κB-Tal-Luc (BD Biosciences) and pGL3-Basic (Promega, Southampton, UK)) were co-transfected with 0.008  μg per well pSV40-Renilla (Promega) DNA, an internal control for normalisation of the transcriptional activity of the reporter vectors.

The luciferase reporter vectors (p κB4-Luc, pCEA205-Luc, p κB4-CEA205-Luc κB4-CEA205-Luc κB4-CEA205-Lucic; 0.8  μg well−1) were co-transfected with 0.008  μg well−1 pSV40-Renilla DNA, an internal control for normalisation of the transcriptional activity of the reporter vectors.

RPL and TUA genes were used as internal controls for normalisation.

For use as an internal control and for normalisation of the results the reference gene ribosomal protein S20 (RPS20) and elongation factor-1α (ELF-1α) were validated for their transcriptional stability in muscle tissue and the RTHDF cells (data not shown).

β-actin (assay ID: 4333762-0711022, Applied Biosystems) was used as the internal control for data normalisation.

Among the more recent ones, Derks et al (2008) showed that HKG expression was not constant enough to be used as internal control for gene normalisation in rat brains.

We investigated the variability of 10 candidate reference genes in D. magna following a 24 h exposure to IB to discover the least variable internal control(s) for QPCR normalisation.